Pichia pastoris HCP ELISA Kit, 2G | F640
- SKU:
- 214-F640
- Size:
- 1 Kit
- Product Type:
- ELISA Kit
- Storage:
- All kit reagents 2°C to 8°C
- Target Expression System:
- P. pastoris
- Species Group:
- Yeast
- Species:
- P. pastoris
- Assay format:
- 96 Well Plate
- Time to result:
- ~3 hrs. 50 min
- LOD:
- ~0.1 ng/mL
- LLOQ:
- ~0.5 ng/mL
- Recommended Diluent:
- I028
Description
Pichia pastoris HCP ELISA Kit, 2G | F640
Pichia pastoris HCP ELISA Kit, 2G is intended for use in determining the presence of Pichia pastoris protein impurities in products manufactured by recombinant expression in Pichia pastoris host cells. Cygnus sells two ELISA kits for Pichia HCP detection. The original kit, Cat# F140, has a very broad reactivity to peptide epitopes and, in addition, also has a small population of antibodies that recognize Pichia glycosylation epitopes. In cases where the recombinant protein product is also glycosylated, there is a potential for some cross reactivity that can result in either an over-estimation of true HCP or indeterminate HCP concentrations due to the lack of sample dilutional linearity. The antibody used in the Cat# F640 kit is the same polyclonal antibody as is used in the F140 kit except it has been subjected to an additional affinity adsorption step to remove antibodies to glycosylation epitopes. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals.
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