Tosoh R40 and R28 Protein A Mix-N-Go ELISA Kit | F910

Tosoh R40 and R28 Protein A Mix-N-Go ELISA Kit is intended for use in quantitating the recombinant Protein A ligands used in the Tosoh Bioscience TOYOPEARL® AF-rProtein A HC-650F (R40) or TOYOPEARL AF-rProtein A-650F (R28) chromatographic resins. When using this assay for TOYOPEARL AF-rProtein A-650F (R28) resin, an adjustment is necessary for assay calibration (see Specificity/Cross Reactivity section). It is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The Mix-N-Go assay for Tosoh R40/R28 Protein A is a two-site immunoenzymetric assay. Samples containing Tosoh R40 or Tosoh R28 Protein A ligands are first diluted in the Mix–N-Go Sample Diluent provided with the kit. The Mix-N-Go Denaturing Buffer is then added and mixed to dissociate the Protein A from the product antibody. The samples are then reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second anti-Protein A antibody labeled directly with Horse Radish Peroxidase (HRP) enzyme is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A:HRP labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with Tetramethylbenzidine (TMB) 800-F910 Mix-N-Go for Tosoh R40/R28 Protein A ELISA Product Insert 2 CYG-LBL-00382 Rev: 01 Eff: 13 Jan 2021 substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Protein A present in the sample. Accurate quantitation is achieved by comparing the signal of unknown samples and controls to Tosoh R40 or Tosoh R28 Protein A Standards assayed at the same time.