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Rubella Elisa IgM capture | M1026

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VRC-M1026
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Description

Rubella Elisa IgM capture | M1026 | Vircell

RUBELLA ELISA

Indirect immunoenzyme assay to test antibodies against rubella virus in human serum/plasma.

  • Serum dilution and sorbent treatment, when necessary, directly in the well
  • The G1026 reference includes calibrators for additional semi-quantification protocol
  • The M1026 reference is based on µ-Capture technique
  • Suitable for automated ELISA systems

Indirect Method - The ELISA method is based upon the reaction of antibodies in the sample tested with the antigen adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. An enzyme-labelled anti-human globulin binds the antigenantibody complex in a second step. After a new washing step, bound conjugate is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.

Capture Method - The ELISA method is based upon the capture of IgM in the sample with anti-IgM antibodies adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. Then the antigen labeled with peroxidase react with the IgM captured, and the unbound is eliminated by washing; bound antigen is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.

  • High performance and guaranteed stability with lyophilized conjugates when necessary.
  • Samples and controls are equally processed to compensate pipetting variability.
  • Colour-coded plates with individual break-apart wells.
  • Coloured, ready-to-use liquid reagents.
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Additional Information

Size:
96 Tests
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