Butorphanol Forensic ELISA Kit | 101119

Neogen's Butorphanol ELISA (Enzyme-Linked ImmunoSorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of Butorphanol. The kit was designed for screening purposes and is intended for forensic use only.

Cross Reactivity

Materials Provided

1. EIA Buffer
2. Wash Buffer Concentrate (10X)
3. K-Blue Substrate
4. Drug-Enzyme Conjugate
5. Antibody Coated Plate
6. Red Stop Solution
7. Qualitative QC Positive Control
8. Qualitative QC Negative Control

Materials Required But Not Provided

1. Deionized water.
2. Precision pipettes that range from 10 μL - 1000 μL and disposable tips.
3. Graduated cylinder to dilute and mix wash buffer.
4. Plate cover or plastic film to cover plate during incubation.
5. Clean glassware (i.e. test tubes) to dilute samples.
6. Microplate reader with 650 nm filter.
7. Cut-off calibrator

Optional Test Materials

1. Qualitative QC Positive Control: 5 x750 μL (synthetic human urine)
2. Qualitative QC Negative Control: 5 x750 μL (synthetic human urine)
3. Red Stop Solution

Optional Materials

1. 1N HCI if Red Stop Solution is not used
2. Microplate shaker