Cotinine Forensic ELISA Kit | 182219

Neogen’s Cotinine ELISA (Enzyme-Linked ImmunoSorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of Cotinine. The Cotinine test kit was designed for screening purposes and is intended for forensic use only.

Cross Reactivity

Materials Provided

1. Wash Buffer Concentrate (10X)
2. K-Blue Substrate
3. Drug-Enzyme Conjugate
4. Drug-Enzyme Conjugate Diluent
5. Antibody Coated Plate
6. Acid Stop Solution
7. QC Positive Control
8. QC Negative Control

Materials Required But Not Provided

1. Deionized water.
2. Precision pipettes that range from 10 μL - 1000 μL and disposable tips.
3. Graduated cylinder to dilute and mix wash buffer.
4. Plate cover or plastic film to cover plate during incubation.
5. Clean glassware (i.e. test tubes) to dilute samples.
6. Microplate reader with 450 nm filter.
7. Cutoff calibrator.
8. Dilution Buffer (1X PBS) for urine samples.
9. NeoSal Buffer for oral fluid samples.
10. EIA Buffer for breast milk samples.

Optional Materials

1. Microplate shaker.