Opiates Hair Forensic ELISA Kit | 140319

Neogen’s Opiates Hair ELISA (Enzyme-Linked Immunosorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of opiates and/or their metabolites in human hair. The kit was designed for screening purposes and is intended for forensic use only.

Materials Provided

1. Wash Buffer Concentrate (10X)
2. K-Blue® Substrate
3. Drug-Enzyme Conjugate
4. Drug-Enzyme Diluent
5. Acid Stop Solution
6. Antibody-coated Plate

1. Dilution Buffer (0.1% BSA/PBS). Dilution Buffer referenced in the Extraction Procedure can be ordered separately (Product no. 301777) or may be made by the laboratory
2. Precision pipettes that range from 10 uL - 1000 uL and disposable tips
3. Graduated cylinder to dilute and mix wash buffer
4. Clean glassware (i.e. test tubes) to complete hair extraction procedure
5. Microplate reader capable of measuring absorbance at 450 nm (650 nm, 630 nm, or 620 nm wavelength reference filter)
6. Borosilicate glass culture tubes or comparable vessels for use with controls, standards and hair extraction procedure. PLASTICS CANNOT BE USED
7. Methanol
8. Heating Block
9. Deionized water.
10. Nitrogen (gas)