ExKine™ Mitochondrion Extraction Kit (Cultured Cells) | KTP4003

Product Overwiew:

Abbkine ExKine™ Mitochondrion Extraction Kit (Cultured Cells) enables isolation of of intact mitochondria from cultured mammalian cells.

Background:

Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.

Used for:

N/A

Kit components:

• Lysis Buffer A (5×)
• Lysis Buffer B
• Lysis Buffer C
• Storage Buffer

Features & Benefits:

• Isolate intact mitochondria from 20 million cells in less than 1 hour.
• Process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method.
• Isolated mitochondria is compatible with almost any downstream application.

Usage Notes:

Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.

Storage buffer:

N/A

Storage instructions:

Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.