Bacillus anthracis RT-PCR (CE) | B101-50FRT

Bacillus anthracis RT-PCR | B101-50FRT from Sacace Biotechnologies is available for delivery

Description:

General information: Real Time PCR test for detection of Bacillus anthracis

Target Disease Type: Dangerous Infections

Specific Application: Bacillus anthracis

Storage and Shipping : on request

Bacillus anthracis RT-PCR (CE) B101-50FRT DataSheet

INTRODUCTION

Bacillus anthracis is a rod-shaped, Gram-positive, aerobic bacterium that is about 1 by 9 micrometers in length. It was shown to cause disease by Robert Koch in 1876. The bacterium normally rests in endospore form in the soil, and can survive for decades in this state. Herbivores are often infected whilst grazing or browsing, especially when eating rough, irritant or spiky vegetation: the vegetation has been hypothesized to cause wounds within the gastrointestinal tract permitting entry of the bacterial endo-spores into the tissues, though this has not been proven. Once ingested or placed in an open cut, the bacterium begins multiplying inside the animal or human and typically kills the host within a few days or weeks. The endo-spores germinate at the site of entry into the tissues and then spread via the circulation to the lymphatics, where the bacteria multiply.

INTENDED USE

The Bacillus anthracis Real-TM PCR kit is an in vitro nucleic acid amplification test for qualitative detection of DNA of vegetative and cryptogamic forms of Bacillus anthracis in biological material and environmental samples and for determination of Bacillus anthracis plasmid composition by identification of pagA (plasmid pXO1) and capA (plasmid pXO2) genes by using real-time hybridization-fluorescence detection.

PRINCIPLE OF ASSAY

Bacillus anthracis Real-TM kit is based on the amplification of pathogen genome specific region using special Bacillus anthracis primers. In real-time PCR, the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes that bind specifically to the amplified product during thermocycling. The real-time monitoring of fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening the reaction tubes after the PCR run. Bacillus anthracis Real-TM kit is a qualitative test that contains the Internal Control (IC). It must be used in the extraction procedure in order to control the extraction process of each individual sample and to identify possible reaction inhibition. Bacillus anthracis Real-TM kit uses “hot-start”, which greatly reduces the frequency of nonspecifically primed-reactions.