DNA-Sorb-B (CE) | K-1-1/B/100

DNA-Sorb-B | K-1-1/B/100 from Sacace Biotechnologies is available for delivery

Description:

General information: DNA extraction kit from whole blood, plasma, liquor, sputum, bioptats, etc.

Target Disease Type: RNA&DNA purification

Specific Application: Silica Sorbtion and Heat-Based* Method

Storage and Shipping : stock

DNA-Sorb-B (CE) K-1-1/B/100 DataSheet

INTENDED USE

The DNA-Sorb-B nucleic acid extraction kit is intended for the isolation and purification of DNA from plasma, serum, whole blood, liquor, amniotic liquid, tissue, urine, feces, bronco aspirates and other biological materials.

STABILITY

DNA-Sorb-B is stable up to the expiration date indicated on the kit label. The product will maintain performance through the control date printed on the label. All components of the DNA-Sorb-B nucleic acid extraction kit are stable until labeled expiration date. The shelf life of reagents before and after the first use is the same, unless otherwise stated. Components stored under conditions other than those stated on the labels may not perform properly and may adversely affect the assay results.

SPECIMEN AND REAGENT PREPARATION

1. Lysis Solution and Washing Solution (in case of their storage at +2-8°C) should be warmed up to 60–65°C until disappearance of ice crystals. Prepare required quantity of 1.5 ml polypropylene tubes including one tube for Negative Control of Extraction.
2. Add to each tube 10 µl of Internal Control (if provided with the amplification kit) and 300 µl of Lysis Solution.
3. Add 100 µl of Samples to the appropriate tube.
4. Prepare Controls as follows: add 100 µl of C– (Neg Control provided with the amplification kit) to the tube labeled Cneg.
5. Vortex the tubes and incubate for 5 min at 65°C. Centrifuge for 7-10 sec. If the sample is not completely dissolved it is recommended to re-centrifuge the tube for 5 min at a maximum speed (12000-16000 g.) and transfer the supernatant into a new tube for DNA extraction.
6. Vortex vigorously Sorbent and add 20 µl to each tube.
7. Vortex for 5-7 sec and incubate all tubes for 3 min at room temperature. Repeat this step.
8. Centrifuge all tubes for 30 sec at 5000g and using a micropipette with a plugged aerosol barrier tip, carefully remove and discard supernatant from each tube without disturbing the pellet. Change tips between the tubes.
9. Add 300 µl of Washing Solution 1 to each tube. Vortex vigorously and centrifuge for 30 sec at 8000g. Remove and discard supernatant from each tube.
10. Add 500 µl of Washing Solution 2 to each tube. Vortex vigorously and centrifuge for 30 sec at 8000g. Remove and discard supernatant from each tube.
11. Repeat step 10 and incubate all tubes with open cap for 5 min at 65°C.
12. Resuspend the pellet in 50 µl of DNA-eluent. Incubate for 5 min at 65°C and vortex periodically.
13. Centrifuge the tubes for 1 min at 12000g.
14. The supernatant contains DNA ready for amplification. If amplification is not performed the same day of extraction, the processed samples can be stored at 2-8°C for at maximum period of 5 days or frozen at –20°/-80°C.