Chlamydia trachomatis/Ureaplasma/M.hominis RT-PCR (CE) | B43-100FRT

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SKU:
441-B43-100FRT
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Test Size:
100
Storage & Shipping:
4 weeks
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Description

Chlamydia trachomatis/Ureaplasma/M.hominis RT-PCR | B43-100FRT from Sacace Biotechnologies is available for delivery

Description:

General information: Multiplex Real Time PCR test

Target Disease Type: Sexually trasmitted diseases

Specific Application: Sexually trasmitted diseases: Multiplex RT-PCR kits

Storage and Shipping : 4 weeks

Chlamydia trachomatis/Ureaplasma/M.hominis RT-PCR (CE) B43-100FRT DataSheet

INTRODUCTION

STDs (sexually transmitted diseases) refer to a variety of bacterial, viral and parasitic infections that are acquired through sexual activity. Some STDs, such as syphilis and gonorrhea, have been known for centuries — while others, such as HIV, have been identified only in the past few decades. STDs are caused by more than 25 infectious organisms. As more organisms are identified, the number of STDs continues to expand. Common STDs include: chlamydia, gonorrhea, herpes, HIV, HPV, syphilis, gardnerella and trichomoniasis.

The Chlamydia trachomatis is nonmotile, gram-negative bacterial pathogen and is the most common sexually transmitted bacterial agent. The prevalence of C. trachomatis infection in sexually active adolescent women, the population considered most at risk, generally exceeds 10%, and in some adolescent and STD clinic populations of women, the prevalence can reach 40%. The prevalence of C. trachomatis infection ranges from 4 to 10% in asymptomatic men and from 15 to 20% in men attending STD clinics. Chlamydial infections in newborns occur as a result of perinatal exposure; approximately 65% of babies born from infected mothers become infected during vaginal delivery.

The development of tests based on nucleic acid amplification technology has been the most important advance in the field of STD diagnosis. Because nucleic acid amplification is exquisitely sensitive and highly specific, it offers the opportunity to use noninvasive sampling techniques to screen for infections in asymptomatic individuals who would not ordinarily seek clinical care.

INTENDED USE

Сhlamydia trachomatis/Ureaplasma/M.hominis Real-TM PCR kit is an in vitro nucleic acid amplification test for multiplex detection of Chlamydia trachomatis, Ureaplasma(parvum and urealyticum) and Mycoplasma hominis DNA in clinical materials (urogenital, rectal and pharyngeal swabs; conjunctival discharge; prostate gland secretion; and urine samples) by using real-time hybridization-fluorescence detection.

PRINCIPLE OF PCR DETECTION

C.trachomatis / Ureaplasma / M.hominis detection by the multiplex polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific C.trachomatis / Ureaplasma / M.hominis primers. In real-time PCR, the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes which bind specifically to the amplified product during thermocycling. The real-time monitoring of the fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening the reaction tubes after the PCR run. Сhlamydia trachomatis/Ureaplasma/M.hominis Real-TM PCR kit is a qualitative test that contains the Internal Control (IC). It must be used in the extraction procedure in order to control the extraction process of each individual sample and to identify possible reaction inhibition.

Сhlamydia trachomatis/Ureaplasma/M.hominis Real-TM PCR kit uses “hot-start”, which greatly reduces frequency of nonspecifically primed reactions. “Hot-start” is guaranteed by chemically modified polymerase (TaqF), which is activated by heating at 95 ºC for 15 min.

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