N.gonorrhoeae/C.trachomatis/ M.genitalium RT-PCR | TB67-100FRT

N.gonorrhoeae/C.trachomatis/ M.genitalium RT-PCR | TB67-100FRT from Sacace Biotechnologies is available for delivery

Multiplex Real Time PCR kit for the detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium

Storage & Shipping :

Stable for up to one year when stored at –20°C, store it at –70°C or below for longer periods.

N.gonorrhoeae/C.trachomatis/ M.genitalium RT-PCR TB67-100FRT DataSheet

INTRODUCTION

STDs (sexually transmitted diseases) refer to a variety of bacterial, viral and parasitic infections that are acquired through sexual activity. Some STDs, such as syphilis and gonorrhea, have been known for centuries — while others, such as HIV, have been identified only in the past few decades. STDs are caused by more than 25 infectious organisms. As more organisms are identified, the number of STDs continues to expand. Common STDs include: chlamydia, gonorrhea, herpes, HIV, HPV, syphilis, gardnerella and trichomoniasis.

The Chlamydia trachomatis is nonmotile, gram-negative bacterial pathogen and is the most common sexually transmitted bacterial agent. The prevalence of C. trachomatis infection in sexually active adolescent women, the population considered most at risk, generally exceeds 10%, and in some adolescent and STD clinic populations of women, the prevalence can reach 40%. The prevalence of C. trachomatis infection ranges from 4 to 10% in asymptomatic men and from 15 to 20% in men attending STD clinics. Chlamydial infections in newborns occur as a result of perinatal exposure; approximately 65% of babies born from infected mothers become infected during vaginal delivery.

The development of tests based on nucleic acid amplification technology has been the most important advance in the field of STD diagnosis. Because nucleic acid amplification is exquisitely sensitive and highly specific, it offers the opportunity to use noninvasive sampling techniques to screen for infections in asymptomatic individuals who would not ordinarily seek clinical care.

INTENDED USE

The kit N.gonorrhoeae/C.trachomatis/M.genitalium Real-TM is a multiplex Real Time PCR test for the qualitative detection of Neisseria gonorrhoeae, Chlamydia trachomatis, and Mycoplasma genitalium DNA in clinical materials (urogenital, rectal, and pharyngeal swabs; conjunctival discharge; prostate gland secretion; and urine samples) by using real-time hybridization-fluorescence detection.

PRINCIPLE OF ASSAY

The kit N.gonorrhoeae/C.trachomatis/M.genitalium Real-TM is based on two major processes: isolation of DNA from specimens and Real Time amplification. DNA is extracted from the specimens, amplified using Real-Time amplification and detected fluorescent reporter dye probes specific for Neisseria gonorrhoeae, Chlamydia trachomatis, and Mycoplasma genitalium DNA and Internal Control. Internal Control (IC) serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition.