N.gonorrhoeae/C.trachomatis/ M.genitalium/T.vaginalis RT-PCR | TB61-100FRT

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SKU:
441-TB61-100FRT
Availability:
Delivery On Request
Test Size:
110
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Description

N.gonorrhoeae/C.trachomatis/ M.genitalium/T.vaginalis RT-PCR | TB61-100FRT from Sacace Biotechnologies is available for delivery

Multiplex Real Time PCR kit for the detection of Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium and Trichomonas vaginalis.

Storage & Shipping :

Stable for up to one year when stored at –20°C, store it at –70°C or below for longer periods.

N.gonorrhoeae/C.trachomatis/ M.genitalium/T.vaginalis RT-PCR TB61-100FRT DataSheet

INTRODUCTION

STDs (sexually transmitted diseases) refer to a variety of bacterial, viral and parasitic infections that are acquired through sexual activity. Some STDs, such as syphilis and gonorrhea, have been known for centuries — while others, such as HIV, have been identified only in the past few decades. STDs are caused by more than 25 infectious organisms. As more organisms are identified, the number of STDs continues to expand. Common STDs include: chlamydia, gonorrhea, herpes, HIV, HPV, syphilis, mycoplasma, gardnerella and trichomoniasis.

The development of tests based on nucleic acid amplification technology has been the most important advance in the field of STD diagnosis. Because nucleic acid amplification is exquisitely sensitive and highly specific, it offers the opportunity to use noninvasive sampling techniques to screen for infections in asymptomatic individuals who would not ordinarily seek clinical care.

INTENDED USE

Kit N.gonorrhoeae/С.trachomatis/M.genitalium/T.vaginalis Real-TM is a multiplex Real Time PCR test for the qualitative detection of Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium and Trichomonas vaginalis in the urogenital swabs, urine, prostatic liquid and other biological materials.

PRINCIPLE OF ASSAY

N.gonorrhoeae/С.trachomatis/M.genitalium/T.vaginalis detection by the multiplex polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific N.gonorrhoeae/С.trachomatis/M.genitalium/T.vaginalis primers. In real-time PCR, the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes which bind specifically to the amplified product during thermocycling.

The real-time monitoring of the fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening the reaction tubes after the PCR run.

N.gonorrhoeae/С.trachomatis/M.genitalium/T.vaginalis Real-TM PCR kit is a qualitative test that contains the Internal Control (IC). It must be used in the extraction procedure in order to control the extraction process of each individual sample and to identify possible reaction inhibition.

N.gonorrhoeae/С.trachomatis/M.genitalium/T.vaginalis Real-TM PCR kit uses “hot-start”, which greatly reduces frequency of nonspecifically primed reactions. “Hot-start” is guaranteed by chemically modified polymerase (TaqF), which is activated by heating at 95 ºC for 15 min.

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1 Review

  • 5

    Excellent

    Posted by Atlas on 7th Jan 2022

    the way Getaur is maintaining its progress and moving on with keeping all the points in mind which can keep their customers happy is excellent

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