Ureaplasma parvum/urealyticum Quant RT-PCR (CE) | B19-100FRT Q

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SKU:
441-B19-100FRT-Q
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Test Size:
100
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Description

Ureaplasma parvum/urealyticum Quant RT-PCR | B19-100FRT Q from Sacace Biotechnologies is available for delivery

Description:

General information: Real Time PCR test for quantitative detect

Target Disease Type: Sexually trasmitted diseases

Specific Application: Ureaplasma species and Mycoplasma

Storage and Shipping : on request

Ureaplasma parvum/urealyticum Quant RT-PCR (CE) B19-100FRT Q DataSheet

INTRODUCTION

STDs (sexually transmitted diseases) refer to a variety of bacterial, viral and parasitic infections that are acquired through sexual activity. Some STDs, such as syphilis and gonorrhea, have been known for centuries — while others, such as HIV, have been identified only in the past few decades. STDs are caused by more than 25 infectious organisms. As more organisms are identified, the number of STDs continues to expand. Common STDs include: chlamydia, gonorrhea, herpes, HIV, HPV, syphilis, gardnerella and trichomoniasis.

Bacterial vaginosis (BV) is a lower genital tract infection characterized by the presence of thin, white, homogeneous, fishy-smelling vaginal discharge. This discharge is present in the absence of signs of vaginal irritation, such as pain, itching, burning, soreness, and dyspareunia. As such, in reference to the lack of demonstrable inflammatory response, the term 'vaginosis' was adopted instead of vaginitis. Bacterial vaginosis is characterized by a disruption of the normal vaginal equilibrium.The lactobacilli population decreases, which leads to an increase in vaginal pH (as high as 7.0) and overgrowth of and replacement by vaginosis-associated anaerobic microorganisms. Up to 90% of women with bacterial vaginosis harbor Gardnerella vaginalis.

Other associated microbial populations identified include Prevotella bivia, Mobiluncus species, Gram-positive cocci, Bacteroides, Mycoplasma hominis, Ureaplasma, Megasphaera, and Leptotrichia. Atopobium vaginae, Streptococcus.

The development of tests based on nucleic acid amplification technology has been the most important advance in the field of STD and BV diagnosis. Because nucleic acid amplification is exquisitely sensitive and highly specific, it offers the opportunity to use noninvasive sampling techniques to screen for infections in asymptomatic individuals who would not ordinarily seek clinical care.

INTENDED USE

Kit U.parvum/U.urealyticum Real-TM Quant is a multiplex Real Time PCR test for the quantitative detection of Ureaplasma parvum and Ureaplasma urealyticum in the urogenital swabs, urine, prostatic liquid and other biological materials.

PRINCIPLE OF ASSAY

U.parvum, U.urealyticum detection by the multiplex polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific regions using specific primers. In real-time PCR, the amplified product is detected using fluorescent dyes. These dyes are linked to oligonucleotide probes which bind specifically to the amplified product during thermocycling. The monitoring of the fluorescence intensities during the real-time PCR allows the detection of accumulating product without re-opening the reaction tubes after the PCR run.

Kit U.parvum/U.urealyticum Real-TM Quant is based on two major processes: isolation of DNA from specimens and Real Time amplification. U.parvum/U.urealyticum Real-TM Quant kit allows to make the quantitative detection in two ways:

  1. Absolute quantification which gives absolute results of copies in 1 ml of clinical sample
  2. Relative quantification which gives the results of the ratio between copies of U.parvum, U.urealyticum and the quantity of human cells. To obtain this result the primers and probes against specific regions of U.parvum and U.urealyticum against human β-globin gene were added to the PCR mix. Furthermore to obtain a quantitative result of human cells, QS standards contain human DNA calibrators. The obtained results of the ratio between the concentration of U.parvum, U.urealyticum and human DNA evaluate the level of the presence of these microorganisms among a population of human cells.
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