Anti 4-hydroxy- 2-hexenal (4-HHE) Monoclonal

4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is highly reactive aldehyde and reacts with histidine residue of protein to form Michael-addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enable to detect HHE-His in the tissue samples.

Clone #:

HHE53

Antigen:

HHE-modified keyhole-lympet hemocyanine

Specificity:

Specific for HHE-modified protein (especially HHE-His Michael adduct)

Subclass:

Mouse IgG1 kappa Prepared as ascite, and protein-A purified.

Form:

Frozen in 10mM PBS containing 0.1% NaN3 and 0.5% BSA.

Application:

Immunohistochemistry; It is recommended that the antibody be tried at 0.5-1.0 µg/mL on paraformaldehyde fixed tissue.

Storage:

Less than -20°C