Histamine ELISA | RE59221

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181-RE59221
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Histamine Antibody | 22939 IHC image of neurons staining for histamine in the rat hypothalamus. The tissue was fixed with 4% carbodimide in 0.05 M phosphate buffered saline, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.
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