Rubella test in S/P IgG/IgM test | RV 128

INTENDED USE:

The Rubella IgG or IgMELISA is intended for use on the detection of IgG or IgM antibody to rubella virus in human serum or plasma.

SUMMARY AND EXPLANATION:

Purified ds-DNA antigen is coated on the surface of microwells. Patient serum or plasma is added to the wells, and the ds-DNA antibody, if present, binds to the antigen during incubation. After washing the wells to remove unbound sample,antibody to human conjugated with HRP is added unbounded conjugate is removed by a subsequent washing step. A solution of TMB Reagent is then added to the microwells. The enzyme conjugate catalytic reaction is stopped at a special time. The intensity of the color generated is proportional to the amount of ds-DNA antibody in the sample.