Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) | 711-545-152

Name: Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) | 711-545-152
SKU: 711-545-152
Price: 240.24 GBP
Availability: InStock
Rating: 4 (1 reviews)

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SKU:: 711-545-152
UPC:
MPN:
Availability:: In Stock
Target:: Rabbit
Host:: Donkey
Antibody Format:: Whole IgG
Specificity:: IgG (H+L)
Minimal Cross Reactivity:: Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, Sheep Serum Proteins
Conjugate:: Alexa Fluor® 488
Clonality:: Polyclonal
Unit:: 0.5 ml

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Description
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Description

Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) | 711-545-152

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Alexa Fluor® 488

Amax: 493 Emax: 519nm

Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.