anti-CD154 | 353-020

Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells.  Interaction of CD154 and CD40 is essential for isotype switching in B cells.  Known genetic defects that alter this interaction lead to impaired immune system function (1).  CD154  has been shown to be hyperexpressed by B and T cells in SLE patients (4).  CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells and macrophages indicating a possible role for the CD40-CD154 immunoregulatory signaling mechanism during inflammation and immunity in atherogenesis (6).

Isotype: Murine IgG1

Immunogen: Human sgp39 fusion protein

Specificity: Antibody 24-31 immunoprecipitates a CD154 (gp39) molecule of about 39 kd.  This clone has been reported to cross react with Cynomolgus and Rhesus Monkey CD154.  Binding avidity of clone 24-31 to CD154 was reported to be 8.5 nM Kd (11).

Functional Application: The antibody 24-31 will block MLR, sgp39 induced human B cell proliferation and T cell dependent B cell differentiation (9).