BrainFast Neuronal Maturation Supplement | BX-2000

BrainFast Neuronal Maturation Supplement is a proprietary combination of small molecules that is used to accelerate the differentiation of neural progenitor cells to mature neurons. It is effective with a variety of neuronal subtypes including, but not limited to, motor neurons, glutamatergic neurons, and GABAergic neurons.

BrainFast Neuronal Maturation Supplement is packaged as two 250 µL vials of 1000X supplement, which is sufficient to treat at least 15 multi-well plates. Begin use after the final plating of differentiating neurons. With two additions (three for long-term cultures) neurons will begin to mature with high penetration and minimal cell death.

CONTENTS

• Two vials of 250 µL BrainFast Supplement
Immediately transfer supplement vials to a -20°C freezer. Once thawed, the supplements can be stored at
4°C for up to one week or aliquotted and returned to -20°C for up to six months.

ADDITIONAL MATERIALS NEEDED

• 5x106 Primary Neurons or Early iPSC- or ESC-Derived Neurons
• DMEM/F12 Medium (Life Technologies #11330-032)
• Neurobasal Medium (Life Technologies #21103-049)
• B27 Supplement (Life Technologies #17504-044)
• N2 Supplement (Life Technologies #17502-048)
• GlutaMAX (Life Technologies #35050-061)
• Geltrex (Life Technologies #A1413201)
• BDNF (Peprotech #450-02)
• GDNF (Peprotech #450-10)
• TGF-β1 (Peprotech #100-21C)
• PDL-Coated 96-Well Plates