Cryopreserved Human Mixed Cortical Neurons | BX-0500

CONTENTS
• One vial of 5 million cryopreserved human neurons (400 μL)
• 200 μL Neuron Seeding Supplement at 1000X
• 100 μL Neuron Day 4 Supplement at 1000X
• 100 μL Supplement C at 2000X

Immediately transfer the vial of neurons from dry ice to liquid nitrogen. Transfer the vials of supplements to a -20°C freezer. Once thawed, the supplements can be stored at 4°C for up to one week.

ADDITIONAL MATERIALS NEEDED
• DMEM/F12 Medium (Life Technologies #11330-032)
• Neurobasal Medium (Life Technologies #21103-049)
• B27 Supplement (Life Technologies #17504-044)
• N2 Supplement (Life Technologies #17502-048)
• GlutaMAX (Life Technologies #35050-061)
• Geltrex (Life Technologies #A1413201)
• BDNF (Peprotech #450-02)
• GDNF (Peprotech #450-10)
• TGF-β1 (Peprotech #100-21C)
• PDL-Coated 96-Well Plates