Description
DAF-2 | D0084
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.
Specifications
- Purity
- ≥95% (HPLC)
- Identity
- 1H-NMR
- Appearance
- Light yellow powder
Properties
- Fluorescence
- λex 505 nm, λem 518 nm in 0.1 M Tris pH 8.0
- Solvents
- DMSO