Goat Anti-AIF1 / IBA1 (isoform 1 and 3) Antibody | EB05419

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SKU:: EB05419
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Availability:: In Stock
Applications:: Pep-ELISA, WB, IHC
Tested species:: Human, Mouse, Rat
Size:: 100µg

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Description
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Description

Goat Anti-AIF1 / IBA1 (isoform 1 and 3) Antibody | EB05419

Immunogen

Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 16-17kDa band observed in Human Frontal Cortex, Rat Brain and in Mouse Brain and lymph node lysates (calculated MW of 16.7kDa according to Human NP_001614.3, 16.9kDa according to Mouse NP_062340.1 and 16.8kDa according to Rat NP_058892.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 4-6µg/ml.