Goat Anti-Doublecortin / DCX (aa232-242) Antibody | EB10781
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- SKU:
- EB10781
- Availability:
- In Stock
- Applications:
- Pep-ELISA, WB, IHC, IF
- Tested species:
- Human, Mouse, Rat
- Size:
- 100µg specific antibody in 200µl
Description
Goat Anti-Doublecortin / DCX (aa232-242) Antibody | EB10781
Target Protein
Principal Names: DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortinOfficial Symbol: DCX
Accession Number(s): NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1
Human GeneID(s): 1641
Non-Human GeneID(s): 13193 (mouse), 84394 (rat)
Important Comments: This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Immunogen
Peptide with sequence C-KTSANMKAPQS, from the internal region of the protein sequence according to NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Mouse NP_001103692.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration: 2-4µg/ml. This antibody has been successfully used in IHC on Rat: Heysieattalab, S et al. (2021) Acta Epileptologica 3, 3 (2021).
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and KNRK cells. Recommended concentration: 5-10µg/ml.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
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