Goat Anti-GAPDH (C Terminus) Loading Control Antibody | EB06377

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SKU:
EB06377
Availability:
In Stock
Applications:
Pep-ELISA, WB, IF, IHC
Tested species:
Human, Rat
Size:
100µg specific antibody in 200µl
  • Goat Anti-GAPDH (C Terminus) Loading Control Antibody | EB06377
  • Goat Anti-GAPDH (C Terminus) Loading Control Antibody | EB06377
  • Goat Anti-GAPDH (C Terminus) Loading Control Antibody | EB06377
  • Goat Anti-GAPDH (C Terminus) Loading Control Antibody | EB06377
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Description

Goat Anti-GAPDH (C Terminus) Loading Control Antibody | EB06377

Target Protein

Principal Names: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase, epididymis secretory sperm binding protein Li 162eP, HEL-S-162eP, peptidyl-cysteine S-nitrosylase GAPDH
Official Symbol: GAPDH
Accession Number(s): NP_002037.2
Human GeneID(s): 2597
Important Comments: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Immunogen

Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 36kDa band observed in lysates of cell line HEK293, approx. 37kDa in Rat Brain lysates and approx.35kDa in Human Liver and Tonsil lysates, lysates of cell line HeLa and in preliminary testing of Mouse Spleen lysates (calculated MW of 36.1kDa according to Human NP_002037.2 and 35.8kDa according to Rat NP_058704.1). Recommended concentration: 0.001-0.003µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Pancreas. Recommended concentration: 2.5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of A549 and HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Dog, Pig
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