Goat Anti-Smooth muscle alpha-actin Antibody | EB06450

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SKU:: EB06450
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Availability:: In Stock
Applications:: Pep-ELISA, WB
Tested species:: Human, Mouse
Size:: 100µg

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Description

Goat Anti-Smooth muscle alpha-actin Antibody | EB06450

Target Protein

Principal Names: actin, alpha 2, smooth muscle, aorta, OTTHUMP00000020042, ACTA2, ACTSA, AAT6, ACTSA, alpha 2 actin, alpha-cardiac actin, growth-inhibiting gene 4, smooth muscle alpha-actin
Official Symbol: ACTA2
Accession Number(s): NP_001604.1; NP_001135417.1
Human GeneID(s): 59
Important Comments: Variants NP_001604.1 and NP_001135417.1 encode the same protein.

Immunogen

Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1; NP_001135417.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 45kDa band observed in Mouse Duodenum lysates and approx 42kDa in lysates of cell lines HeLa and NIH3T3 (calculated MW of 42kDa according to Human NP_001604 and Mouse NP_031418.1). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature.