HemaVision® -7 Positive Controls | HV05-7PC

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HV05-7PC
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Description

HemaVision® -7 Positive Controls | HV05-7PC

HemaVisionÒ-7PC is a CE-marked in vitro diagnostic kit containing 10 tubes with in vitro transcribed
RNA diluted into wild type leucocyte RNA from the cell line HL60. The 10 in vitro transcribed RNA
originates from transcription of 10 fusion gene constructs. These 10 tubes with of in vitro transcribed
RNA mixed with HL60 total RNA can be used as positive controls for the 7 translocations detected by
the HemaVisionÒ-7 kit. Three translocations t(9;22), t(15;17), inv(16) have breakpoints separated by
several hundreds of base pairs. Consequently, each of these three translocations are covered by two in
vitro transcribed RNA. The HL60 RNA contains transcripts of the Biotinidase (BTD) gene which are
detected by reaction control primers of the HemaVisionÒ kits.
The HemaVisionÒ single translocation kits Cat No. HV03-119, HV03-411, HV03-821, HV03-922, HV03-
1221, HV03-1517, HV03-inv16 are identical with corresponding split-out PCR primer mixes from the
HemaVisionÒ-7 kit. Therefore, the 10 positive control RNA mixes in HemaVisionÒ-7PC can also be used
to test the single translocation kits.

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