IL-33 (oxidation resistant) (human) (rec.) (untagged) | AG-40B-0160-C010

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SKU:
AG-40B-0160-C010
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Package:
10 µg
£289.43

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Description

IL-33 (oxidation resistant) (human) (rec.) (untagged) | AG-40B-0160-C010

Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released upon cell lysis. IL-33 binds to and signals through ST2 (IL-1R1) and its stimulation recruits MYD88, IRAK, IRAK4 and TRAF6, followed by phosphorylation of ERK1 (MAPK3) / ERK2 (MAPK1), p38 (MAPK14) and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases and sepsis. IL-33 facilitates Treg expansion in vitro and in vivo. Recently, IL-33 has been involved in adipocyte differentiation. The biological activity of IL-33 at its receptor ST2 is rapidly terminated in the extracellular environment by its oxidation (formation of two disulfide bridges), resulting in an extensive conformational change that disrupts the ST2 binding site. Mutations at amino acids C208S/C232S protect IL-33 from oxidation and increase its activity.

Properties
Source/Host E. coli
Sequence

Human IL-33 (aa 112-270) is untagged. Amino acids C208 and C232 have been mutated to serine to protect IL-33 from oxidation.

Crossreactivity Human
Mouse
Specificity

Binds to human and mouse ST2.

Biological Activity

Activates human and mouse ST2-dependent NF-κB pathway. Activates in vivo Innate Lymphoid Cells 2 (ILC2) at 0.4µg /ml.

MW ~17kDa (SDS-PAGE); Monomer (Size Exclusion Chromatography)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 100µg size: 1mg/ml
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS + 1mM DTT
 
 
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