• Extensively validated according to FDA/ICH/EMEA guidelines
• Correlates with existing methods
• One-step ELISA

Intact FGF23 ELISA Product Overview

The human intact FGF23 ELISA Kit is a one-step, 96-well sandwich ELISA for the quantitative determination of intact FGF 23 in human plasma. TheFGF-23 ELISA employs human plasma-based standards to ensure the measurement of biologically reliable data.

Intact FGF23 ELISA Assay Principle

The human FGF 23 intact ELISA kit is a sandwich enzyme immunoassay that has been optimized and validated for the quantitative determination of intact FGF23 in human plasma samples. Serum and other sample types are compatible with this ELISA.

Figure explaining the principle of the human intact FGF-23 ELISA kit:

Capture antibody: monoclonal recombinant anti-human FGF23 antibody
Detection antibody: monoclonal mouse anti-human FGF23 antibody, HRP-labeled
Target antigen: human intact FGF23

In a first step, standard/control/sample and conjugate (monoclonal mouse anti-human FGF23-HRP) are pipetted into the wells of the microtiter strips, which are pre-coated with monoclonal recombinant anti-human FGF23 antibody. FGF23 present in the standard/control/sample binds to the pre-coated antibody in the well and forms a sandwich with the conjugated anti-human FGF23-HRP antibody. In the washing step all non-specific unbound material is removed. In a second step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of intact FGF23 present in the sample. This color change is detectable with a standard microplate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) versus standard concentration is generated using the values obtained from the standards. The concentration of intact FGF23 in the sample is determined directly from the dose response curve.

Principle of the Detection of intact FGF23

The FGF23 (intact) human ELISA is a sandwich-based immunoassay with a recombinant monoclonal anti-human FGF23 capture antibody recognizing a structural epitope in the N-terminal part of FGF23. Detection is mediated by a HRP-labeled monoclonal anti-human FGF23 antibody directed to the C-terminal part of FGF23. Therefore, specific measurement of intact FGF23 is ensured.

Intact FGF23 ELISA Typical Standard Curve

The figure below shows a typical standard curve for the human FGF23 ELISA. The immunoassay is calibrated against recombinant human intact FGF23:

Intact FGF23 ELISA Kit Components