The Ki-67 protein is a nuclear protein doublet, 345-395 kDa, playing a pivotal role in maintaining cell proliferation. In
diagnostic histopathology and cell biology, the antibody has proven valuable for the demonstration of the Ki-67 antigen in
normal and neoplastic cells, for example in soft-tissue sarcoma, prostatic adenocarcinoma, and breast carcinoma. The Ki-67
has been confirmed as a very powerful single prognostic factor for overall survival, with highly proliferative cases showing a
much poorer outcome than tumors with low proliferation. In breast cancer, the proliferative index measured by Ki-67
immunoreactivity has both prognostic and predictive value.

Intended use: For Research Use Only
Reactivity: Human
Clone: MIB-1
Species of origin: Mouse
Isotype: IgG1 k
Control Tissue: Tonsil
Staining: Nuclear
Presentation: Tissue culture supernatant containing 15mM sodium azide
Application and suggested dilutions:
Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0 for 20 minutes is required for IHC staining on
formalin-fixed, paraffin embedded tissue sections.
• Immunohistochemical staining of formalin-fixed, paraffin embedded tissue section (dilution 1:400-1:800)
The optimal dilution for a specific application should be determined by the investigator.
Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based
detection is recommended.