Description

Mini Samples ELISA Kit for Erythropoietin (EPO) | MEA028MU

Name: Mini Samples ELISA Kit for Erythropoietin (EPO) | MEA028MU
SKU: MEA028MU
Availability: OutOfStock

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Erythropoietin (EPO).
No significant cross-reactivity or interference between Mini Samples Erythropoietin (EPO) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Erythropoietin (EPO) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Erythropoietin (EPO) in samples.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Erythropoietin (EPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Erythropoietin (EPO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.