As one of the cyclin-dependent kinase inhibitors that inhibit cylcin-dependent kinases 4 and 6, p16INK4A is encoded by tumor
suppressor gene CDKN2A. The tumor suppressor p16INK4A plays an important role in cell cycle regulation. Increased
expression of the p16 gene, which is seen as organisms age, reduces the proliferation of stem cells. This reduction in the
division and production of stem cells protects against cancer while increasing the risks associated with cellular senescence.
Mutations in the p16 gene associated with loss or over expression of the protein are associated with increased risk of a
wide range of cancers and cancer precursor lesions. The Immunohistochemical identification of p16 is particularly relevant
in uterine cervical lesions: Development of dysplasia is closely related to human papilloma virus (HPV) infection.

Intended use: For Research Use Only
Reactivity: Human
Clone: MX007
Species of origin: Mouse
Isotype: IgGK
Control Tissue: Human cervical cancer, tonsil
Staining: Cytoplasmic and Nuclear
Presentation: Tissue culture supernatant containing 15mM sodium azide
Application and suggested dilutions:
Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on
formalin-fixed, paraffin embedded tissue sections.
• Immunohistochemical staining of formalin-fixed, paraffin embedded tissue section
(dilution 1:100 - 1:400)
The optimal dilution for a specific application should be determined by the investigator.
Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based
detection is recommended.
Storage & Stability: Store at 2-8 C. Do not use after expiration date printed on the vial.