SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein | 40592-V08H

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SKU:
40592-V08H
Availability:
In Stock
Size:
100 µg
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Description

SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein | 40592-V08H

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (RBD, His Tag)(Cat: 40592-V08H), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (RBD, His Tag)(Cat: 40592-V08H) is 20-60 ng/mL.
Protein Construction
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus.
Expressed Host
HEK293 Cells
Species
SARS-CoV-2
Predicted N Terminal
Arg319
Molecule Mass
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 26.54 kDa.
Formulation
Lyophilized from sterile PBS, PH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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