Features

• European Union: for in vitro diagnostic use
• Rest of the world: for research use only!
• The total assay time is less than 3 hours
• The kit measures total soluble transferrin receptor in serum and plasma (EDTA, citrate, heparin)
• Assay format is 96 wells
• Quality Controls are human serum based. No animal sera are used
• Standard is natural human blood isolated sTfR based
• Components of the kit are provided ready to use or concentrated

Research topic

Iron metabolism, Oncology

Summary

The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.05–2 µg/ml

Limit of Detection

2 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.8%

Inter-assay (Run-to-Run)

n = 4; CV = 6.3%

Spiking Recovery

100,20%

Dilution Linearity

94,00%

Crossreactivity