Blocking one | 03953-95

Blocking one | 03953-95

Blocking is indispensable in immunoassays in order to block non-specific binding reactions. Blocking One contains both high molecular weight compounds and bovine serum protein. Blocking One is superior to conventional blocking solutions. The preservative in Blocking One does not affect the enzyme activity of peroxidase (POD) or alkaline phosphatase (ALP). Only simple refrigerator storage is necessary, even after opening the bottle.

Comparison of blocking reaction times

The relationship between reaction time and the effect of blocking in microplate assay. In comparison with other products, Blocking One shows by far the fastest dinamics.

Since the antigen reaction is omitted in this experiment, when staining occurs, it is caused by non-specific adsorption. The figure shows negative data

Applications

Linearity in quantitative protein analysis

This product does not contain preservative which affects the enzyme activity of Peroxidase (POD) or Alkaline Phosphatase (ALP). Using common enzyme (POD, ALP) gives good result with high linearity.

Detection of Mouse IgG(H+L) by Western Blotting

Prevention of non-specific antibody binding in immunohistochemical application

Histological staining of normal organ tissue slices had been performed based on the following protocol, and the efficiency of different blocking agents wrere compared (20 minutes blocking time). The dots stained brown show Fibronectin existence. The key for successful observation is the contrast between the stained dots and the background, Blocking One gives the lowest background.