C.L.E.D. with Andrade's Indicator | DM111

C.L.E.D. with Andrade's Indicator | DM111
A modified C.L.E.D. medium for the investigation of urinary infections.

In an attempt to combine the advantages of the C.L.E.D medium described by Mackey and Sandys (1966)1 with the ease of
differentiation of MacConkey's medium, Bevis (1968)¨ proposed the addition to C.L.E.D. medium of acid fuchsin. This modification, on which the MAST medium is based, made it easier to identify colonial types and differentiate between lactose and nonlactose fermenters. Mixed cultures of different organisms are more obvious, and tests such as coagulase, oxidase, catalase and agglutination with specific sera, can be performed on primary isolates from this medium.

2. Technical Formula*

3. Directions
1. Suspend by swirling 35.2g of powder in
1 litre or the contents of the sachet in the stated amount of distilled or deionised water.
2. Autoclave at 121°C (15p.s.i.) for 15 minutes.
3. Thoroughly mix before pouring

4. In Use
MAST C.L.E.D. with Andrade's Indicator can be used in conjunction with MAST Bacteruritest Strips (BTR1) for the screening of urine cultures. Incubation of plates at 37°C should be continued for 18-24 hours but no longer, otherwise the medium may begin to turn pink. If use of a CO2 incubator is envisaged, the final pH of the medium should be adjusted to over 7.5.