DNA Damage Quantification Colorimetric Kit | K253-25
- SKU:
- K253-25
- Availability:
- 5 Working days
- Detection Method:
- Absorbance (450 and 650 nm)
- Species Reactivity:
- Mammalian
- Storage Conditions:
- -20°C
- Shipping Conditions:
- Gel Pack
- USAGE:
- For Research Use Only! Not For Use in Humans.
Description
DNA Damage Quantification Colorimetric Kit
Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed duringthe course of base excision and repair of oxidized, deaminated or alkylated bases. It hasbeen estimated that about 2x105 base lesions are generated per cell per day. The level ofAP sites in cells can be a good indicator of DNA lesion and repair against chemicaldamage and cell aging. The DNA Damage Quantification Kit utilizes the ARP (AldehydeReactive Probe) reagent that reacts specifically with an aldehyde group which is the openring form of the AP sites. After treating DNA containing AP sites with ARP reagents, APsites are tagged with biotin residues, which can be quantified using avidin-biotin assayfollowed by a colorimetric detection. The kit provides the necessary reagents forconvenient determination of abasic sites in purified DNA sample in 96-well plate format.
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