FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit | E-EL-0009

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SKU:: E-EL-0009
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Availability:: 5 Working days
Assay type:: Competitive-ELISA
Format:: 96T/48T
Assay time:: 2.0h
Detection range:: 1.56-100 ng/mL
Sensitivity:: 0.94 ng/mL
Sample type &Sample volume:: Serum, plasma and other biological fluids; 50μL
Specificity:: This kit recognizes FA/VB9 in samples. No significant cross-reactivity or interference between FA/VB9 and analogues was observed
Reproducibility:: Both intra-CV and inter-CV are < 10%
Application:: This ELISA kit applies to the in vitro quantitative determination of FA/VB9 concentrations in serum, plasma and other biological fluids.

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Description
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Description

FA/VB9 (Folic Acid/Vitamin B9) ELISA Kit | E-EL-0009

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type & Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes FA/VB9 in samples. No significant cross-reactivity or interference between FA/VB9 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of FA/VB9 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with FA/VB9. During the reaction, FA/VB9 in the sample or standard competes with a fixed amount of FA/VB9 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to FA/VB9. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FA/VB9 in tested samples can be calculated by comparing the OD of the samples to the standard curve

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level FA/VB9 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level FA/VB9 were tested on 3 different plates, 20 replicates in each plate.