Mouse LDL ELISA Kit | MBS2501275

LDL elisa kit :: Mouse LDL ELISA Kit | MBS2501275

Intended use
This ELISA kit applies to the in vitro quantitative determination of Mouse LDL concentrations in serum, plasma and
other biological fluids.
Specification
●Sensitivity: 4.69ng/mL.
●Detection Range: 7.81-500ng/mL
●Specificity: This kit recognizes Mouse LDL in samples. No significant cross-reactivity or interference between Mouse
LDL and analogues was observed.
●Repeatability: Coefficient of variation is < 10%.
Test principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with
an antibody specific to Mouse LDL. Standards or samples are added to the micro ELISA plate wells and combined with
the specific antibody. Then a biotinylated detection antibody specific for Mouse LDL and Avidin-Horseradish Peroxidase
(HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The
substrate solution is added to each well. Only those wells that contain Mouse LDL, biotinylated detection antibody and
Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop
solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The OD value is proportional to the concentration of Mouse LDL. You can calculate the concentration of
Mouse LDL in the samples by comparing the OD of the samples to the standard curve.
Kit components & Storage
An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately
according to the following conditions once the kit is received.