AffiGEL® Matrix LDEV-Free Equivalent to: CORNING Matrigel Cat No. 356234 & 354234

AffiGEL® Matrix LDEV-Free

Equivalent: 356234 & 354234 | Corning® Matrigel® Basement Membrane Matrix, LDEV-free

Matrix type: Basic concentration (8-12 mg/ml)

Application: Adapt to 2D and 3D culture, invasion, and migration experiments, and can also be used for in vivo tumorigenic experiments.

Product Information 

AffiGEL® Matrix LDEV-Free is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

• Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations are determined by Lowry method
• Endotoxin units are measured by Limulus Amoebocyte Lysate assay
• AffiGEL® Matrix Gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of AffiGEL® Matrix Gel and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor.

Source

Engelbreth-Holm-Swarm Mouse Tumor

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.

Application

• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• in vitro and in vivo Angiogenesis Assays
• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

AFG-YSG-01

AFG-YSG-02