Accumax™

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SKU:
17087-54
Availability:
5 to 7 Days Shipment
Size:
100 ml
Storage Conditions:
Freeze
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Description

Accumax™ | 17087-54from Nacalai Tesque is available for delivery.

Accumax is a ready to use non-mammalian, non-bacterial replacement for all applications of trypsin and collagenase in tissue dissociation, cell counting, and dissolving cell clumps such as spheroids. Accumax contains proteolytic and collagenolytic enzymes, and can be used to create single cell suspensions from clumped cell cultures and to dissociate spheroids..

Features

  • Dissociates tissue.
  • Increases accuracy of manual or automated cell counts.
  • Used to dissolve neuronal and prostate spheroids.
  • Removes cells from 3-D matrixes.
  • Removes cells from hollow fiber cell reactors.
  • Extends the sort time of clumpy cell on a sorting flow cytometer.

Cell Lines Cryopreserved with Accumax

A few cell lines that Accumax has been shown to work on without harm:

  • hESCs
  • fibroblasts
  • keratinocytes
  • vascular endothelial cells
  • hepatocytes
  • vascular smooth muscle cells
  • hepatocyte progenitors
  • primary chick embryo neuronal cells
  • bone marrow stem cells
  • adherent CHO cells
  • adherent BHK cells
  • macrophages
  • 293 cells
  • L929 cells
  • immortalized mouse testicular germ cells
  • 3T3
  • Vero
  • COS
  • HeLa
  • NT2
  • MG63
  • M24
  • A375 metastatic melanoma
  • gliomas U251
  • gliomas D54
  • HT1080 fibrosarcoma cells
  • Sf9 insect cells

Applications

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