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CBB Stain One (Ready To Use)

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SKU:
04543-51
£402.00

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Description

CBB Stain One (Ready To Use) | 04543-51 from Nacalai Tesque is available for delivery.

Product Information

CBB Stain One is ready-to-use Coomassie Brilliant Blue G-250 staining solution for protein electrophoresis.

Application 1

Gel stained with CBB Stain One

Electorophoresis conditions

  • Gel : 12% acrylamide gel
  • Sample : protein MW markers, GST-tag fusion protein

After electrophoresis, the gel was rinsed with de-ionized water to remove SDS, then stained with CBB Stain One for 1 hr.Clear bands were obtained by de-staining with de-ionized water.

Data courtesy of Dr.Yoshida K., Department of Molecular Genetics,Medical Research Institute, Tokyo Medical and Dental University

Application 2

Conditions

Gel : ReadyGel 4-20%
Buffer solution : Tris/Glycine/SDS buffer solution (400ml)
Electrophoresis conditions : 80mA (constant voltage)
Wash : water (50ml) 3 times
Staining time : 1 hr.

Sample

Lane 1 : E.coli proteins (SDS, with 2ME buffer extraction) 5 ml
Lane 2 : E.coli proteins (SDS, with 2ME buffer extraction) (10 time dilution) 5 ml
Lane 3 : protein markers (1.7 mg/ml) 2 ml
Lane 4 : protein markers (550 ng/ml) 2 ml
Lane 5 : protein markers (170 ng/ml) 2 ml
Lane 6 : protein markers (55 ng/ml) 2 ml

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