5-HIAA (5-Hydroxyindoleacetic Acid) Antibody | 24274

5-HIAA (5-Hydroxyindoleacetic Acid) Antibody | 24274 | ImmunoStar

The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilution of the antiserum is 1/4000-1/8000 for biotin-streptavidin/HRP technique. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.

Host: Rabbit

State: Lyophilized Whole Serum

Reacts With: Rat, Tritonia Diomedea (Sea Slug)

Alternate Names: anti-5-HIAA

RRID: AB_572208

Immunogen: 5-HIAA

Gene Symbol: SLC6A4

Gene ID: 6532

NCBI Gene Aliases: 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, hSER