5-HT (Serotonin) Transporter Antibody | 24330

5-HT (Serotonin) Transporter Antibody | 24330 | ImmunoStar

The ImmunoStar serotonin (5HT) transporter was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/10,000 – 1/15,000 in PBS/0.3% Triton X-100 – Biotin/avidin-HRP. By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. The predicted molecular weight of serotonin transporter based on its nucleic acid sequence is 67,815. The actual weight that one might observe from tissue extracts could be higher due to glycosylation. We validated the antibody with rat SERT overexpressed in an insect cell expression system. The MW from that was about 67 kD. Our SERT antibody dilution was 1:20,000 using chemiluminescent detection. Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter (602-622).

Host: Rabbit

State: Lyophilized Whole Serum

Availability: In Stock

Reacts With: Human, Marine, Monkey, Mouse, Rat, Sparrow

Alternate Names: 5HTT; HTT; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; Slc6a4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4

RRID: AB_572209

Immunogen: Rat 5-HT transporter (579-599)

Gene Symbol: Slc6a4