Insulin Antibody | 20056

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SKU:
484-20056
Quantity/Volume:
100 µL
  • Insulin Antibody | 20056
  • IHC image of beta cells in the islets of Langerhans of the rat pancreas staining for insulin and counterstained with hemotoxylin . The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen
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Description

Insulin Antibody | 20056 | ImmunoStar

The ImmunoStar Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/3000-1/5000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.

Host: Rabbit

State: Lyophilized Whole Serum

Reacts With: Bubalus Bubalis (Buffalo), Cattle, Chick, Cow, Heifer (Cow), Horse, Human, Lizard, Macaque, Manducta Sexta (Moth), Monkey, Mouse, Pig, Rat, Sheep

Availability: In Stock

Alternate Names: anti-Insulin

Gene Symbol: INS

RRID: AB_572246

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