Neuropeptide Y Y1 Receptor Antibody | 24506

(No reviews yet) Write a Review
SKU:
484-24506
Quantity/Volume:
100 µL
  • Neuropeptide Y Y1 Receptor Antibody | 24506
  • IHC image of the rat cortex staining for the neuropeptide Y Y1 recptor. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host: Rabbit
Select Currency //481.00

 Select your currency from the header

Description

Neuropeptide Y Y1 Receptor Antibody | 24506 | ImmunoStar

The ImmunoStar Neuropeptide Y Y1 Receptor was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 – 1/1000 in PBS – Bn/Av-HRP detection. The three black and white images are confocal image of spinal cord neurons labeled with antibody 24506 against the neuropeptide Y Y1 receptor using immunofluorescence. More details in Marvizon et al., 2019, Neuropharmacology 158: 107732. Courtesy of Juan Carlos Marvizon, Ph.D., UCLA. The antibody was characterized by immunohistochemistry and Western blot. Western blot showed one immunoreactive band of 40 kD and a single high molecular weight band, presumably a precursor molecule. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human NPY Y1 receptors.

State: Liquid

Reacts With: Hamster, Human, Mouse, Rat

Availability: In Stock

Alternate Names: Neuropeptide Y receptor 1; NPY receptor 1; NPYR; NPYY1; NPY1-R; Neuropeptide Y receptor type 1; FC5; NPYR1, neuropeptide Y1 receptor, anti-NPYY1

Gene Symbol: neuropeptide Y receptor Y1, anti-NPYY1

RRID: AB_572257

View AllClose

0 Reviews

View AllClose

Related Products

Neuropeptide Y Antibody | 22940 IHC image of neurons staining for neuropeptide Y in the rat cortex and striatum . The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The second image above entitled “C.” is NPY interneurons (RED) in mouse striatum, GREEN staining is GFP expressed by medium spiny neurons provided Alipi Naydenov, Neurobiology and Behavior, Stella Lab University of Washington (see review below).
Add to Cart
Quick view

Neuropeptide Y Antibody | 22940

Select Currency //379.00
Neuropeptide Y Antibody | 22940 | ImmunoStarNeuropeptide Y (NPY) is a member of a regulatory peptide family and has a marked sequence homology with pancreatic polypeptide (PP) and peptide YY (PYY),...
484-22940