Gellan Gum

A plant tissue culture gelling agent for preparing clear, solid or semi-solid culture media.

Product Overview

Gellan Gum is an anionic polysaccharide used as a gelling agent and as an alternative to agar in plant tissue culture and selected microbiological media. Its repeating tetrasaccharide structure contains two D-glucose residues, one L-rhamnose residue and one D-glucuronic acid residue.

In plant tissue culture, Gellan Gum can produce a firm, relatively transparent gel that makes it easier to observe plant tissues and root development. Depending on the culture medium and protocol, it may be used at a lower concentration than agar.

Key Features

Applications

Gellan Gum is used as a gelling component in plant culture media and selected microbial culture workflows.

• Plant Tissue Culture: Solid or semi-solid media for in vitro plant cultivation.
• Micropropagation: Culture media for plant multiplication and regeneration.
• Plant Regeneration: Gel support for cultured plant tissues and explants.
• In Vitro Plant Culture: Controlled cultivation of shoots, roots and plant tissues.
• Plant Biotechnology: Research involving plant propagation and tissue culture.
• Culture Medium Preparation: Can be incorporated into suitable basal media, including MS and DKW formulations.
• Microbial Culture: Can be used as a solidifying agent in appropriate microbial culture media.

Example: Preparation of 1 L Plant Tissue Culture Medium

The following is a general preparation outline. Follow the validated protocol for your basal medium and culture system.

1. Add approximately 800 mL of distilled water to a suitable beaker.
2. Add 4.54 g of MS basal medium (or an appropriate alternative) and 30 g of sugar.
3. Add PPM™ if required by the selected protocol; a typical example is 1–2 mL/L.
4. Bring the solution to a final volume of 1,000 mL.
5. Adjust the pH to approximately 5.4–5.8.
6. Add 3–4 g/L of Gellan Gum while stirring.
7. Autoclave using a validated cycle. A commonly used cycle is 121°C, 15 psi for 20 minutes, subject to the medium and vessel load.
8. Allow the medium to cool and solidify before use.

Note: Medium composition, pH, gelling-agent concentration and sterilization conditions should be optimized for the plant species and laboratory protocol.

Features & Benefits

• Easy to incorporate into suitable culture media.
• Provides solid support for explant growth and development.
• Allows plant tissues to access nutrients within the culture medium.
• Transparent gel helps visualize root development and possible contamination.
• May offer a cost-effective alternative to agar, depending on the formulation.
• Supports preparation of solid or semi-solid media for laboratory plant culture.

Product Specifications

Available Sizes

Gellan Gum is listed in the following pack sizes. Availability may vary.

This product listing is for the 100 g size. Contact Gentaur to inquire about other pack sizes.

Frequently Asked Questions

Looking for Gellan Gum for Your Laboratory?

Contact Gentaur for product information, availability and ordering assistance.