Gibberellic Acid (GA3)

A plant growth regulator used in plant tissue culture, seed germination and plant growth research.

Product Overview

Gibberellic Acid (GA3), also known as Gibberellin A3, is a plant hormone that plays an important role in plant growth and development. This tetracyclic diterpenoid compound is involved in processes such as stem elongation, seed germination, flowering and fruit development.

In plant tissue culture, GA3 may be added to suitable growth media to influence cell elongation and support the development of shoots and young plant tissues. Its effects depend on the plant species, culture conditions and concentration used.

Key Features

Applications

Gibberellic Acid (GA3) is used in plant science research and agricultural applications involving plant growth and development.

• Plant Tissue Culture: Added to suitable culture media to influence shoot and tissue development.
• Seed Germination: Used in research on germination and seed dormancy.
• Cell Elongation: Supports studies of stem elongation and plant tissue growth.
• Plant Regeneration: Used in selected in vitro plant growth and regeneration protocols.
• Flowering and Fruit Development: Investigated for its role in flowering, fruit growth and crop development.
• Agricultural Research: Used in studies involving crop growth and plant development.
• Barley Malting: Gibberellins may be used to stimulate the germination-related processes of dormant barley kernels.

General Tissue Culture Media Preparation

GA3 is commonly incorporated into plant tissue culture media from a prepared plant growth regulator (PGR) stock solution. The appropriate concentration depends on the plant species and experimental protocol.

1. Prepare liquid tissue culture medium using MS, DKW or another suitable basal medium.
2. Add sugar according to the selected formulation. A common example is 30 g/L.
3. If required, add PPM™ according to the laboratory protocol.
4. For solid media, add a suitable gelling agent, such as 3–4 g/L Gellan Gum or 6–8 g/L agar, as a starting formulation.
5. Prepare or use a suitable GA3 stock solution and add the required amount to achieve the desired final concentration.
6. Mix thoroughly and sterilize the medium using a validated protocol appropriate for the formulation.

Features & Benefits

• Helps promote seed germination and break dormancy in suitable plant systems.
• Supports research into stem elongation and cell growth.
• Involved in cell division and fruit development processes.
• Useful for plant tissue culture and plant growth research.
• Powder format for preparation of laboratory stock solutions.
• Suitable for tissue culture applications.

Product Specifications

Frequently Asked Questions

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