NuGel™ Poly-Hydroxy

NuGel™ Poly-Hydroxy For Immobilization

• Non-specific sites are virtually eliminated by a polymer coating
• Stable across a wide pH range 2 – 10
• 1000Å, 50µm Silica suitable for LC and batch processes
• Applications include: Enzyme immobilization, separation and purification of biopolymers, immobilization of proteins & ligands – monoclonal antibodies, hormones, peptides, haptens, drugs, etc

Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. With NuGel™, non-specific sites have been virtually eliminated making it an ideal support for affinity purification. Through a proprietary polymer coating, Silica is crosslinked forming a reactive Poly-Epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.

NuGel™ Poly-Hydroxy Technical Data

NuGel™ Polyhydroxy support has a terminal glycol group(cis-diol). This alpha glycol group can be activated either by sodium-m-periodate to form active aldehyde groups at the end or by a variety of leaving groups such as carbonyldiimidazole, chloroformates, sulfonates, etc. These leaving groups are easily replaced by nucleophilic ligands containing amino and thiol groups.

Special Features:

• Couples ligands containing free terminal glycol groups in the presence of a Carbonyl diimidazole.
• Couples non-polar ligands in organic solvents.
• pH stable from 2 to 9.

Click Here To View NuGel™ Poly-Hydroxy Product Sheet