Streptavidin Conjugated with Horseradish Peroxidase (SA-HRP)

Horseradish streptavidin conjugated peroxidase (HRP streptavidin, streptavidin HRP, sa-hrp, hrp-sa, etc.) is a kind of high purity streptavidin (SA) bound with high purity and purified horseradish peroxidase. It can be used to detect biotinylated antibodies, proteins, tissues, cells, nucleic acid and other samples. It is often used as a signal amplifier to improve detection sensitivity.

Streptavidin (SA) is a tetrameric protein formed by four identical subunits, with a molecular weight of about 60kDa, which can bind to biotin with high specificity, and one SA can bind to four biotins. Compared to egg white avidin, SA has no glycosylation and its isoelectric point is neutral or slightly acidic, so its nonspecific binding is much weaker than avidin, and its nonspecific background is very low when used for detection.

Horseradish peroxidase (HRP) is a type of glycoprotein containing heme. Its molecular weight is approximately 40 kDa, the sugar content is 18%, brown. It can be incubated with substrate to produce some kind of molecular derivative labeled by staining, fluorescence or luminescence. This phenomenon makes it widely used in the detection of molecular biology, biochemistry, immunology and other fields.

The following table is the recommended dilution ratio for conventional use. In the actual test run, the dilution ratio of sa-hrp can be adjusted appropriately depending on the specific experimental conditions. Using the example of the conventional western bolt, 10 ml of 1: 2000 diluent is required for each test, and only 5 μL (5 μg) is required for each test.

BM: Western bolt;

IHC: Immunohistorichemistry; IC: Immunocytochemistry.

For research use only!

Streptavidin Conjugated with Horseradish Peroxidase (SA-HRP) DataSheet