Sabouraud Dextrose Agar with Chloramphenicol | NCM0068

Sabouraud Dextrose Agar with Chloramphenicol | NCM0068

Intended Use
Sabouraud Dextrose Agar W/ Chloramphenicol is used for the selective isolation of fungi in a laboratory
setting. Sabouraud Dextrose Agar W/ Chloramphenicol is not intended for use in the diagnosis of
disease or other conditions in humans.
Description
Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. Sabouraud
Dextrose Agar is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose
concentration and acidic pH of the formula permits selectivity of fungi. This medium is beneficial in
sporulation studies and pigment production. Sabouraud Dextrose Agar is used for determining the
microbial content of cosmetics and in the mycological evaluation of food.
Sabouraud Dextrose Agar W/ Chloramphenicol is a modification of Sabouraud Dextrose Agar, with the
addition of Chloramphenicol to increase selectivity against commensal microorganisms.
Typical Formulation
Enzymatic Digest of Casein 5.0 g/L
Enzymatic Digest of Animal Tissue 5.0 g/L
Dextrose 40.0 g/L
Chloramphenicol 0.05 g/L
Agar 15.0 g/L
Final pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Precaution
Refer to SDS
Preparation
1. Suspend 65 g of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
Quality Control Specifications
Dehydrated Appearance: Powder is homogeneous, free flowing, and light beige.
Prepared Appearance: Prepared medium is clear to slightly hazy and light beige to amber.
Expected Cultural Response: Cultural response on Sabouraud Dextrose Agar W/ Chloramphenicol at
25 - 30°C and examined for growth after 2 - 7 days of incubation. 

Test Procedure
Consult appropriate references for recommended test procedures on the isolation and identification of yeast and molds.
Results
Yeasts grow creamy to white colonies. Molds will grow as filamentous colonies of various colors. Refer to appropriate references for a complete discussion on yeast and molds.
Expiration
Refer to expiration date stamped on the container. The dehydrated medium should be discarded if not free flowing, or if the appearance has changed from the original color. Expiry applies to medium in its intact container when stored as directed.
Limitations of the Procedure
1. Some strains may be encountered that grow poorly or fail to grow on this medium.
2. Antimicrobial agents incorporated into a medium to inhibit bacteria may also inhibit certain pathogenic fungi.
3. This medium is sensitive to over-heating, due to the low pH, and could cause agar to soften.
Storage
Store sealed bottle containing the dehydrated medium at 2 - 30°C. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed.